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CHI TIẾT
Xây dựng phương pháp định lượng đồng thời methylisotriazolinon (MI) và methylchloroisotriazolinon (MCI) trong mẫu khăn ướt bằng kỹ thuật HPLC
Development of an HPLC method for simultaneous determination of methylisotriazolinon (MI) và methylchloroisotriazolinon (MCI) in marketed wet paper tissues
Lê Đình Chi, Nguyễn Văn Trung, Lê Thị Hường Hoa
Số: 518 - Tháng 6/2019 - Trang 13-16

Mỹ phẩm là những sản phẩm dễ bị phân hủy bởi tác nhân lý hóa cũng là môi trường thuận lợi cho vi khuẩn phát triển. Vì vậy, các nhà  sản xuất thường cho thêm chất bảo quản vào mỹ phẩm để ngăn chặn quá trình nhiễm khuẩn, giúp sản phẩm không bị phân hủy và kéo dài thời gian sử dụng. Chất bảo quản hay sử dụng trong mỹ phẩm là các paraben và các chất thuộc nhóm isothiazolinon. methylchlorotriazolinone (MCI) và methylisotriazolinone (MI) là 2 chất bảo quản thuộc nhóm isothiazolinon gây dị ứng, trong đó  MCI gây dị ứng mạnh nên không được sử dụng đơn độc. Châu Âu, các nước ASEAN và Cục Quản lý Dược Việt Nam qui định giới hạn của MCI và MI như sau: Không quá 100 ppm cho MI, không quá 15 ppm cho hỗn hợp MCI/MI với tỷ lệ 3:1. Đã sử dụng hỗn hợp MCI/MI thì không được có thêm MI.

            Hiện nay theo hệ thống hòa hợp ASEAN thì chưa đề cập đến phương pháp định lượng đồng thời 2 chất bảo quản MCI và MI. Trong bài báo này, xin giới thiệu kết quả nghiên cứu định lượng đồng thời MCI và MI trong khăn ướt bằng phương pháp sắc ký lỏng hiệu năng cao pha đảo với detector PDA.

Nguyên liệu

            - Chất đối chiếu: MI (BCBN3373V; SKS: 0103170; hàm lượng 98,2 % (nguyên trạng); MCI (SKS: 8-XAL-159-1; hàm lượng 96,0 % (nguyên trạng) (Sigma-Aldrich, Singapore).

Đối tượng nghiên cứu

            - Mẫu không chứa MCI và MI phục vụ xây dựng thẩm định phương pháp: Khăn ướt Luck-lady do Công ty TNHH Đầu tư và Thương mại Đông Hiệp sản xuất.

            - Mẫu khăn ướt mua ngẫu nhiên trên thị trường.

Phương pháp:

- Điều kiện sắc ký

- Chuẩn bị mẫu

+ Dung dịch chuẩn gốc MI

+ Dung dịch chuẩn gốc MCI

+ Dung dịch chuẩn hỗn hợp

+ Dung dịch thử

+ Dung dịch đánh giá (mẫu tự tạo)

Kết luận

Trong nghiên cứu này, một phương pháp HPLC đơn giản đã được xây dựng và áp dụng để kiểm tra hai chất bảo quản chính nhóm isotriazolinon là MI, MCI trong sản phẩm khăn ướt. Phương pháp đã được thẩm định đạt các yêu cầu về hiệu năng của phương pháp phân tích theo yêu cầu của AOAC International, qua đó chứng tỏ sự phù hợp với mục đích ứng dụng dự kiến. Kết quả phân tích bước đầu trên một số mẫu khăn ướt trên thị trường đã phát hiện thấy trường hợp sử dụng MI, MCI ở mức vượt quá quy định cho phép, có thể ảnh hưởng tới sức khỏe người dùng.

An HPLC method was developed for simultaneous determination of methylisotriazolinone (MI) and methylchlorotriazolinone (MCI) in wet paper tissues. The injection sample was made by methanolic extraction. The method was established as Column - C8 (250 x 4.6 mm, 5 mm); Detector – PDA (274 nm); Mobile phase - a mixture of methanol – water (30 : 70, v/v); Flow rate - isocratic, 1.0 ml/min; Injection volume - 20 ml. The method was validated in terms of specificitiy, system suitability, precision, accuracy, linearityrang, limit of quantitation and limit of detection; all proved the developed method simple, reliable and suitable for the intended application. As for practical application, various commercial samples of wet paper tissue were tested revealing a potential risk of using MI, MCI as preservatives at higher levels than legally permitted.

Quay trở lại

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